prevalence of herpes simplex virus type-1, 2 and varicella zoster virus (vzv) in eye infection
نویسندگان
چکیده
abstract background and aims: herpes simplex virus (hsv) is a common cause of corneal disease and is the leading infectious cause of corneal blindness among developed nations. this study is aimed to provide an estimation of the incidence of herpes simplex virustype-1, 2(hsv1, 2) and varicella zoster virus (vzv) in tears and swab from eye infection by polymerase chain reaction (pcr) in eye disease in a suspected community. materials and methods: fifty subjects without signs of ocular herpetic disease enrolled in the study. serum samples from all subjects were tested for hsv igg antibodies by enzyme-linked immunosorbent assay (elisa). subjects were instructed to collect tear samples by touching the inner surface of the lower eyelid with an individually wrapped, sterile cotton swab and to place the swab immediately into labeled sterile tubes. swabs were kept at 4°c until processed. nucleic acid was extracted from the samples and pcr-amplified for hsv dna. results: among 50 samples, 3 samples were refused because internal controls were negative. hsv infection was established in 10% (5 out of 50) of all patients. the prevalence of hsv infection in patients with no clinical suspicion of herpetic keratitis was 6%. histopathologic evaluation revealed that among samples with positive pcr results, 100% had evidence of inflammation, 55% had giant cells, 39% had necrosis, 59% had vascularization, 67 % had ulcer and 100% of them had inclusion bodies. conclusions: because some of the patients with no clinical suspicion of herpes infection were found positive, we suggest that hsv to be considered as one of the underlying etiologies in any patient with corneal scar and keratitis. therefore, performing further diagnostic methods, including pcr and histopathology, is mandatory to clearly understand the infection.
منابع مشابه
Prevalence of Herpes Simplex Virus type-1, 2 and Varicella Zoster Virus (VZV) in Eye Infection
Abstract Background and Aims: Herpes simplex virus (HSV) is a common cause of corneal disease and is the leading infectious cause of corneal blindness among developed nations. This study is aimed to provide an estimation of the incidence of Herpes Simplex Virustype-1, 2(HSV1, 2) and Varicella Zoster Virus (VZV) in tears and swab from eye infection by polymerase chain reaction (PCR) in eye ...
متن کاملprevalence of herpes simplex virustype-1, 2 and varicella zoster virus (vzv) in eye infection
background and aims: herpes simplex virus (hsv) is a common cause of corneal disease and is the leading infectious cause of corneal blindness among developed nations. this study is aimed to provide an estimation of the incidence of herpes simplex virustype-1, 2(hsv1, 2) and varicella zoster virus (vzv) in tears and swab from eye infection by polymerase chain reaction (pcr) in eye disease in a s...
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There are six herpes viruses, three of which, the varicella-zoster virus and the herpes simplex viruses types 1 and 2, are of particular concern to patients and staff in critical care units. These viruses, especially in their reactivated states, may present atypically in critically ill and immune-suppressed patients, and, by the time the diagnosis is made, exposures of other patients and clinic...
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Herpes simplex virus type 1 (HSV-1) and varicella zoster virus (VZV) are closely related viruses causing lifelong infections. They are typically associated with mucocutaneous or skin lesions, but may also cause severe neurological or ophthalmic diseases, possibly due to viral- and/or host-genetic factors. Although these viruses are well characterized, genome-wide evolutionary studies have hithe...
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background: seroepidemiologic studies indicate a high prevalence of herpes simplex virus (hsv) infection. this infection leads to ophthalmic, dermatologic, oral, neurologic, vaginal and cervical problems. different studies have been carried out to estimate the hsv seroprevalence in iran. combining the results of these studies would be useful for health policy-making.objective: this study aims t...
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عنوان ژورنال:
iranian journal of virologyجلد ۹، شماره ۳، صفحات ۶-۱۴
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